After completing nearly 6 years of graduate work, Erica has decided she should start running Northern blots. ("Again!", she adds.) While her project has been mainly concerned with protein-protein interactions, her committee members decreed that since she works in an RNA lab ("Technically!", Erica interjects), there should be more Northern blots in her thesis.
Unfortunately, as it's been more than 4 years since she ran her last Northern, Erica found she had a little trouble pouring such a large gel.
"You mean I have to pour it into the gel tray? Not right into the buffer circulation tank?"
Better luck next time, Miss Champion.
Don't worry, you still don't have to give RNA seminar.
